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Immunolabelling for Protein Localization

immunolabelling for protein localization"Tokuyasu-style" cryosections

This method consists of sectioning samples in aqueous solution at cryogenic temperature samples.

While the ultrastructure is not as good as the epoxy-resin embedding method, proteins maintain better antigenicity and can be detected by immunolabeling using antibodies conjugated to gold nanoparticles.

This technic is suitable for protein localization and counting on electron micrographs.

 

IF on cryosectionsWe also perform immunofluorescence on semithin cryosections. While this is useful to identify regions of interest in tissues and cell pellets prior to immunocytochemistry, the images produced are extremely sharp, as the fluorophore signal is not blurred by out-of-focus fluorescence.